Semiquantitative plate assay for determination of cellulase production by Trichoderma viride

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7323954

Montenecourt, B S; Eveleigh, D E

A plate clearing assay was devised to screen for high-producing cellulase mutants of Trichoderma viride. The method employs (i) the use of either rose bengal or oxgall to limit colony size and (ii) Phosfon D (tributyl-2,4-dichlorobenzylphosphonium chloride) to enhance cellulase detection, in combination with acid-swollen cellulose on agar plates. The method was used to isolate constitutive cellulase mutants of T. viride and should prove useful for isolating high-producing mutants from a range of organisms. This technique has been also used to determine the concentration at which glucose and glycerol inhibit cellulase synthesis by catabolite repression in the wild-type strains.

• Research Organization: Rutgers--The State Univ., New Brunswick, NJ
• OSTI ID: 7323954
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 33:1; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
BIOASSAY
BIODEGRADATION
BIOMASS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CULTURES
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
DYES
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYME INHIBITORS
ENZYMES
FUNGI
GLUCOSE
GLYCEROL
GROWTH
HEXOSES
HYDROLYSIS
HYDROXY ACIDS
HYDROXY COMPOUNDS
INDICATORS
INHIBITION
LYSIS
MONOSACCHARIDES
MUTANTS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PLANTS
POLYSACCHARIDES
POPULATIONS
REAGENTS
RENEWABLE ENERGY SOURCES
REPLICA TECHNIQUES
ROSE BENGAL
SACCHARIDES
SCREENS
SIZE
SOLVOLYSIS
SYNTHESIS