Pollen mitosis and pollen tube growth inhibition by SO/sub 2/ in cultured pollen tubes of Tradescantia
Mature pollen of Tradescantia paludosa Anders, Sax clone-3 was collected from greenhouse grown plants and desiccated in the dark for 4--5 hours. The pollen was sown on microslides coated with lactose agar medium (supplemented with 0.02 percent colchicine). Five minutes after sowing, pollen cultures were treated with various concentrations (0.075--2000 ppM) of SO/sub 2/ obtained from the chemical reaction between K/sub 2/S/sub 2/O/sub 5/ and HCl. Treatments were carried out in an airtight Plexiglas chamber throughout the full incubation period of 19 hours. Treated and control pollen cultures were fixed and stained in aceto-carmine or Feulgen reaction to determine the tube lengths and mitotic indices. Results of repeated experiments indicated that the values of mitotic indices dropped from the normal 38.7 percent in the control to the range of 24.3--3.8 percent when treated with 0.075--50.00 ppM of SO/sub 2/. Pollen tube growth was inhibited from the normal length of 1.5 mm in the control to the range of 800--100 ..mu..m when treated with 10--2000 ppM of SO/sub 2/. The inhibitory effect of SO/sub 2/ presumably resulted from physiological disturbance of the cell and severe damage to chromosomes of both generative and tube nuclei of the pollen tubes.
- Research Organization:
- Western Illinois Univ., Macomb
- OSTI ID:
- 7310750
- Journal Information:
- Environ. Res.; (United States), Vol. 12:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
A COMPARISON OF X-RAY AND ULTRAVIOLET INDUCED ABERRATIONS IN POLLEN TUBE CHROMOSOMES OF TRADESCANTIA. Final Report
THE INFLUENCE OF OXTGEN ON THE SENSITIVITY OF TRADESCANTIA POLLEN TUBE CHROMOSOMES TO X-RAYS
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
GROWTH
INHIBITION
POLLEN
MITOSIS
SULFUR DIOXIDE
BIOLOGICAL EFFECTS
AGAR
ANAEROBIC CONDITIONS
CARMINIC ACID
CELL CULTURES
CHROMOSOMAL ABERRATIONS
COLCHICINE
CULTURE MEDIA
CYTOCHEMISTRY
DRYING
FEULGEN METHOD
HERBS
HISTOLOGICAL TECHNIQUES
HYDROCHLORIC ACID
INCUBATION
LACTOSE
MITOTIC INDEX
PLANTS
STAINS
SULFUR COMPOUNDS
ALKALOIDS
ANTHRAQUINONES
ANTIMITOTIC DRUGS
AROMATICS
BIOMASS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL DIVISION
CHALCOGENIDES
COLLOIDS
DISACCHARIDES
DISPERSIONS
DRUGS
ENERGY SOURCES
GAMETES
GERM CELLS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INORGANIC ACIDS
MUTATIONS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLYSACCHARIDES
QUINONES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SULFUR OXIDES
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)
550400 - Genetics