Regulation of two extracellular proteases of Neurospora crassa by induction and by carbon-nitrogen and sulfur-metabolite repression

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(75)90347-1· OSTI ID:7309647

Cohen, B L; Morris, J E; Drucker, H

Synthesis and release by Neurospora crassa 74A (FGSC 262) of a neutral and an alkaline protease have been studied by experiments in which mycelia grown in Vogel's minimal medium were transferred to media containing protein inducer and deficient in carbon, nitrogen, and/or sulfur sources. The kinetics of accumulation of each of the two proteases in filtrates of induced, metabolite-deprived (derepressed) cultures were found to be similar and the same two electrophoretically separable enzymes were elicited by each of the three induction and derepression treatments. Moreover, antiserum specific for the major protease (Protease 2) elicited by one of the treatments gave a reaction of identity with the major proteases elicited by the other two treatments. It would therefore appear that these two proteases are coordinately regulated by a single system of induction and repression in which successful induction by exogenous protein depends upon the lifting of any one of carbon, nitrogen, or sulfur metabolite repressions.

• Research Organization: Pacific Northwest Labs., Richland, WA
• OSTI ID: 7309647
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 169; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BIOMASS
BIOSYNTHESIS
ELECTROPHORESIS
ENERGY SOURCES
ENZYMES
FUNGI
HYDROLASES
IMMUNE SERUMS
METABOLISM
NEUROSPORA
PEPTIDE HYDROLASES
PLANTS
RENEWABLE ENERGY SOURCES
SYNTHESIS