Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays
An endonuclease which is active upon DNA exposed to ultraviolet light at a photoproduct other than thymine dimers has been extensively purified from Escherichia coli. The small (2.7 S) enzyme is active in the presence of EDTA, has a neutral pH optimum, and is inhibited by tRNA and 1 M NaCl. It has no detectable exonuclease, DNA N-glycosidase, or ribonuclease activities. The enzyme also nicks duplex DNA exposed to OsO/sub 4/, x-rays, or acid, but it does not act upon undamaged DNA or irradiated single-stranded DNA. The majority of sites of action in DNA exposed to ultraviolet light or OsO/sub 4/ appear to be alkali-stable, but those in DNA exposed to x-rays or acid are not. The incisions created by the endonuclease contain 5'-phosphate termini. The enzyme is possibly the same as E. coli endonuclease III described by Radman but it is distinguishable from the other endodeoxyribonucleases described from that organism.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 7306927
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 252:9; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CHALCOGENIDES
CHELATING AGENTS
DIMERS
DNA
EDTA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OSMIUM COMPOUNDS
OSMIUM OXIDES
OXIDES
OXYGEN COMPOUNDS
PH VALUE
PHOSPHOTRANSFERASES
PURIFICATION
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RNA
RNA-ASE
THYMINE
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
ULTRAVIOLET RADIATION
URACILS
X RADIATION