Soman and glutamate toxicity in cultured cortical and hippocampal neurons: An in vitro model for testing neuroprotective drugs
An in vitro mammalian model neuronal system to evaluate intrinsic toxicity of soman and other neurotoxicants and the efficacy of potential countermeasures was developed. Primary dissociated cell cultures from rat hippocampus and cerebral neocortex have been established. The link between soman toxicity, glutamate hyperactivity and neuronal death in the central nervous system was investigated. The cytotoxicity was assessed by trypan blue dye exclusion and also from the measurement of LDH released by damaged cells in the extracellular fluid 24 hr after exposure. Cortical or hippocampal cells exposed to glutamate for 15 min caused neuronal death in almost 80 % of the cells examined at 24 hr. when cortical cells were exposed to soman alone for 15 to 120 min, washed to remove excess ChE inhibitor and incubated for 24 hr, no damage was evident as assessed by trypan blue exclusion or LDH assay. Soman does not appear to have direct toxic effect on the cerebral cortical neurons.
- Research Organization:
- Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
- OSTI ID:
- 7300607
- Report Number(s):
- AD-P-008804/7/XAB
- Resource Relation:
- Other Information: This article is from 'Proceedings of the Medical Defense Bioscience Review (1993) Held in Baltimore, Maryland on 10-13 May 1993. Volume 2', AD-A275 668, p483-492
- Country of Publication:
- United States
- Language:
- English
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