Bovine lymphocytic leukemia: studies of etiology, pathogenesis and mode of transmission. Progress report No. 17, July 1976--October 1977

Technical Report · Fri Jul 22 04:00:00 EDT 1977

DOI:https://doi.org/10.2172/7300348· OSTI ID:7300348

Sorensen, D. K.

The primary objective of the proposed research will be elucidation of the etiology and pathogenesis of bovine leukemia. We have consistently demonstrated C-type particles in mitogen stimulated lymphocyte cultures from leukemic cows and cows with a persistent lymphocytosis. These particles have been concentrated and partially purified by continuous flow, density gradient, ultracentrifugation. Newborn calves and late stage bovine fetuses have been inoculated with these concentrated cell free preparations. Our current study involves extensive monitoring of these inoculated animals to detect early pre-cancerous changes. The following parameters are being measured: the serological titer against a bovine leukemia associated antigen; the percentage of lymphocytes showing nuclear pockets; the percentage of mitogen stimulated lymphocytes with C-type particles adherent to their surface; the percentage of B-lymphocytes in the peripheral circulation; the complete blood count; and the quantity of bovine leukemia virus (BLV) production as determined by the syncytia induction assay. Additional proposals include: using the monitoring parameters to study animals with the juvenile and thymic forms of leukemia; the examination of adult lymphosarcoma cases to determine which tissues harbor BLV; and lymphocyte subpopulation work to further define which cell types are associated with BLV production and tumor formation.

• Research Organization: Minnesota Univ., St. Paul (USA). Coll. of Veterinary Medicine
• OSTI ID: 7300348
• Report Number(s): COO-910-43
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATTLE
CONNECTIVE TISSUE CELLS
DISEASES
DOMESTIC ANIMALS
ETIOLOGY
FETUSES
HEMIC DISEASES
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
LYMPHOMAS
LYMPHOSARCOMAS
MAMMALS
MICROORGANISMS
MONITORING
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
PARTICLES
PATHOGENESIS
RUMINANTS
SARCOMAS
SOMATIC CELLS
VERTEBRATES
VIRUSES