Characterization of cyanogen bromide peptides of hypoxanthine phosphoribosyltransferase by a micromethod. [/sup 125/I, /sup 3/H, /sup 35/S tracer techniques]
Journal Article
·
· Anal. Biochem.; (United States)
Human hypoxanthine phosphoribosyltransferase (HPRT-EC 2.4.2.8), either from erythrocytes labeled in vitro with /sup 125/I or from HeLa cells labeled in vivo with (/sup 3/H)leucine, (/sup 3/H)lysine, or (/sup 3/H)arginine, was cleaved with cyanogen bromide. All four labeled enzymes produced cyanogen bromide peptides with molecular weights of 7800, 5600, 4100, 2500, and 1300 which were separated by sodium dodecyl sulfate urea polyacrylamide gel electrophoresis. Experiments with (/sup 35/S)methionine-labeled HPRT indicated that 88 to 94% of the /sup 35/S label was released by the cyanogen bromide reaction. The relative distribution of radioactivity in each of the tritiated peptides indicates that there are 20 leucines, 15 lysines, and 12 arginines per enzyme subunit.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 7294629
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 82:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400100 -- Analytical & Separations Chemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ARGININE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CLEAVAGE
CRYSTAL STRUCTURE
CYANOGEN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
HELA CELLS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
HYPOXANTHINE
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUCINE
LIGHT NUCLEI
LYSINE
MICROSTRUCTURE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PROTEINS
PURINES
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TRANSFERASES
TRITIUM
YEARS LIVING RADIOISOTOPES
400100 -- Analytical & Separations Chemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ARGININE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CLEAVAGE
CRYSTAL STRUCTURE
CYANOGEN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
HELA CELLS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
HYPOXANTHINE
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUCINE
LIGHT NUCLEI
LYSINE
MICROSTRUCTURE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PROTEINS
PURINES
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TRANSFERASES
TRITIUM
YEARS LIVING RADIOISOTOPES