Toxic effect of metals on DNA synthesis
Technical Report
·
OSTI ID:7294451
An in vitro DNA polymerase I system was used to study the effects of divalent metal ions on DNA replication/repair. The metals Ba/sup + +/, Ca/sup + +/, Cu/sup + +/, Co/sup + +/, Ni/sup + +/, Cd/sup + +/, Hg/sup + +/, Pb/sup + +/, and Zn/sup + +/ increasingly inhibited DNA polymerization in the order listed. BSA had a profound effect in the system, abolishing the inhibitory effect of Cd/sup + +/. DNA primer-template at sufficiently high levels abolished Cd/sup + +/ inhibition. Adjustment of mononucleotide levels gave rise to a multiphasic inhibition pattern. The interpretation of these findings with respect to toxicity levels of divalent metals is discussed.
- Research Organization:
- California Univ., Los Angeles (USA)
- DOE Contract Number:
- EY-76-C-03-0012
- OSTI ID:
- 7294451
- Report Number(s):
- UCLA-12-1130
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATOMIC IONS
BARIUM IONS
BIOSYNTHESIS
CADMIUM IONS
CALCIUM IONS
CHARGED PARTICLES
CHEMICAL REACTIONS
COBALT IONS
COPPER IONS
DNA
ELEMENTS
INHIBITION
IONS
LEAD IONS
MERCURY IONS
METALS
NICKEL IONS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYMERIZATION
SYNTHESIS
TOXICITY
ZINC IONS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATOMIC IONS
BARIUM IONS
BIOSYNTHESIS
CADMIUM IONS
CALCIUM IONS
CHARGED PARTICLES
CHEMICAL REACTIONS
COBALT IONS
COPPER IONS
DNA
ELEMENTS
INHIBITION
IONS
LEAD IONS
MERCURY IONS
METALS
NICKEL IONS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYMERIZATION
SYNTHESIS
TOXICITY
ZINC IONS