Organization of repeated nucleotide sequences in the replicons of mammalian DNA. [X Radiation]

Journal Article · Mon Aug 01 04:00:00 EDT 1977 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(77)85574-4· OSTI ID:7291135

Mattern, M R; Painter, R B

Chinese hamster ovary cells were irradiated with 100 to 5,000 rads of x-rays, and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was compared with that of DNA synthesized during the 60 min after sham irradiation and with that of parental DNA. Nascent DNA from cells whose replicon initiation was inhibited renatured faster than nascent DNA from control cells in the C/sub 0/t range of repeated nucleotide sequences, suggesting that regions of the replicon not close to origins are enriched in repeated sequences and that regions close to origins are enriched in unique sequences. A class of repeated nucleotide sequences may be involved in the regulation of replicon initiation.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 7291135
• Journal Information: Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 19:2; ISSN BIOJA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
DNA
ELECTROMAGNETIC RADIATION
HAMSTERS
IONIZING RADIATIONS
KINETICS
MAMMALS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RODENTS
SYNTHESIS
VERTEBRATES
X RADIATION