Repair of DNA in xeroderma pigmentosum conjunctiva. [UV radiation]
Journal Article
·
· Arch. Ophthalmol. (Chicago); (United States)
Xeroderma pigmentosum (XP) is an autosomal recessive disease with tumor formation on sun-exposed areas of the skin and eyes. Cells from most XP patients are deficient in repairing DNA damaged by ultraviolet (uv) light as shown by a reduced rate of tritiated thymidine (3HTdR) incorporation during their DNA repair synthesis. We have studied such repair synthesis in conjunctival cells from an XP patient with a conjunctival epithelioma and from normal cadaver conjunctiva. Cultured conjunctival cells were irradiated with uv light and then incubated with 3HTdR. Autoradiograms were prepared and showed that uv radiation induced a considerably slower rate of DNA repair synthesis in the XP cells than in normal cells. Many of the ocular abnormalities of XP, including tumor formation, may be the result of this defective DNA repair process.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 7282500
- Journal Information:
- Arch. Ophthalmol. (Chicago); (United States), Journal Name: Arch. Ophthalmol. (Chicago); (United States) Vol. 93:8; ISSN AROPA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genetic complementation analysis of xeroderma pigmentosum
Ultraviolet light-resistant primary transfectants of xeroderma pigmentosum cells are also DNA repair-proficient
DNA single-strand breaks during repair of uv damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum
Conference
·
Tue Dec 31 23:00:00 EST 1974
·
OSTI ID:4145053
Ultraviolet light-resistant primary transfectants of xeroderma pigmentosum cells are also DNA repair-proficient
Journal Article
·
Tue Aug 15 00:00:00 EDT 1989
· Biochemical and Biophysical Research Communications; (USA)
·
OSTI ID:5601875
DNA single-strand breaks during repair of uv damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum
Journal Article
·
Wed Dec 31 23:00:00 EST 1975
· Proc. Natl. Acad. Sci. U.S.A., v. 73, no. 1, pp. 39-43
·
OSTI ID:4093474
Related Subjects
560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BODY
CELL CULTURES
CONJUNCTIVA
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EYES
MUCOUS MEMBRANES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SENSE ORGANS
SKIN
SKIN DISEASES
SYNTHESIS
ULTRAVIOLET RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BODY
CELL CULTURES
CONJUNCTIVA
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EYES
MUCOUS MEMBRANES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SENSE ORGANS
SKIN
SKIN DISEASES
SYNTHESIS
ULTRAVIOLET RADIATION