Kinetic investigation of microbial souring in porous media using microbial consortia from oil reservoirs

Journal Article · Fri Jul 01 00:00:00 EDT 1994 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260440302· OSTI ID:7271306

Chen, C I; Reinsel, M A; Mueller, R F ^[1]

---

1. Montana State Univ., Bozeman, MT (United States). National Science Foundation Engineering Research Center for Biofilm Engineering

Microbial souring (H[sub 2]S production) in porous media was investigated in an anaerobic upflow porous media reactor at 60 C using microbial consortia obtained from oil reservoirs. Multiple carbon sources (formate, acetate, propionate, iso and n-butyrates) found in reservoir waters as well as sulfate as the electron acceptor were used. Kinetics and rates of souring in the reactor system were analyzed. Higher volumetric substrate consumption rates (organic acids and sulfate) and a higher volumetric H[sub 2]S production rate were found at the front part of the reactor column after H[sub 2]S production had stabilized. Concentration gradients for the substrates (organic acids and sulfate) and H[sub 2]S were generated along the column. Biomass accumulation throughout the entire column was observed. The average specific sulfate reduction rate (H[sub 2]S production rate) in the present reactor after H[sub 2]S production had stabilized was calculated to be 11.62 [+-] 2.22 mg sulfate-S/day g biomass.

• OSTI ID: 7271306
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 44:3; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROGEN SULFIDES
BIOSYNTHESIS
SULFATE-REDUCING BACTERIA
GROWTH
SULFATES
METABOLISM
BIOREACTORS
OIL FIELDS
RESERVOIR FLUIDS
SOUR CRUDES
BACTERIA
CHALCOGENIDES
ENERGY SOURCES
FLUIDS
FOSSIL FUELS
FUELS
GEOLOGIC DEPOSITS
HYDROGEN COMPOUNDS
MICROORGANISMS
MINERAL RESOURCES
OXYGEN COMPOUNDS
PETROLEUM
PETROLEUM DEPOSITS
RESOURCES
SULFIDES
SULFUR COMPOUNDS
SYNTHESIS
020300* - Petroleum- Drilling & Production
560300 - Chemicals Metabolism & Toxicology