Stable enzyme inhibitors and stable free radical species in ultraviolet-irradiated solutions of chlorpromazine

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Mol. Pharmacol.; (United States)

OSTI ID:7267084

Goucher, C; Windle, J J; Levy, L

Irradiated solutions of chlorpromazine inhibited the activities of yeast alcohol dehydrogenase (alcohol : NAD/sup +/ oxidoreductase, EC 1.1.1.1), beef heart lactate dehydrogenase (L-lactate : NAD/sup +/ oxidoreductase, EC 1.1.1.27), glyceraldehyde phosphate dehydrogenase (D-glyceraldehyde-3-phosphate : NAD/sup +/ oxidoreductase (phosphorylating), EC 1.2.1.12), and uridine diphosphoglucose dehydrogenase (uridine diphosphate glucose : NAD/sup +/ oxidoreductase, EC 1.1.1.22) even after long periods of storage in darkness at 40/sup 0/. Two classes of products were obtained from irradiated chlorpromazine. Solutions of these products both inhibited enzyme activities and contained relatively stable free radicals. During and following free radical decay, the inhibitory activity of the solutions containing them was essentially unchanged. It is concluded that stable photolytic products in these solutions, other than free radicals, contributed predominantly to the inhibition of enzyme activity observed.

• OSTI ID: 7267084
• Journal Information: Mol. Pharmacol.; (United States), Journal Name: Mol. Pharmacol.; (United States) Vol. 11:5; ISSN MOPMA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AZINES
CHEMICAL REACTIONS
CHLORPROMAZINE
DECOMPOSITION
DISPERSIONS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
HETEROCYCLIC COMPOUNDS
INHIBITION
IRRADIATION
MIXTURES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTHIAZINES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PSYCHOTROPIC DRUGS
RADIATIONS
RADICALS
SOLUTIONS
TRANQUILIZERS
ULTRAVIOLET RADIATION