Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.10.4576· OSTI ID:7266927

Tomavo, S; Schwarz, R T; Dubremetz, J F ^[1]

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1. Institut National de la Recheche Medicale, Villeneuve d'Ascq (France)

The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with {sup 3}H-fatty acids, ({sup 3}H)ethanolamine, and ({sup 3}H)carbohydrates. Treatment of {sup 3}H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.

• OSTI ID: 7266927
• Journal Information: Molecular and Cellular Biology; (USA), Vol. 9:10; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
LABELLING
CARBOHYDRATES
CARBOXYLIC ACIDS
ELECTROPHORESIS
GLUCOSAMINE
LIPASES
MANNOSE
MICE
PARASITES
PROTOZOA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
AMINES
ANIMALS
CARBOXYLESTERASES
ENZYMES
ESTERASES
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
HYDROLASES
INVERTEBRATES
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques