Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries
- Ames Lab., IA (United States)
Various excitation schemes for distributing a laser beam to a large number of capillaries in an array are evaluated. This led to the construction of a multiplexed system for monitoring the electrophoresis of DNA fragments in 100 capillaries. The laser-excited fluorescence signals from each capillary are simultaneously recorded at the rate of 0.6 frame/s by a CCD camera. The reconstructed electropherograms show excellent reproducibility and minimal cross-talk. The system provides for two simultaneous excitation wavelengths so that it can be adapted for two-color, two-intensity DNA sequencing based on the commercial four-dye chemistry. Only 20 mW per laser line was employed. Further development of this system to accommodate up to 4096 independent sequencing channels at a time is discussed. 26 refs., 6 figs., 2 tabs.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 7265367
- Journal Information:
- Analytical Chemistry (Washington); (United States), Vol. 66:9; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
DNA SEQUENCING
MONITORING
CAMERAS
CAPILLARIES
CHARGE-COUPLED DEVICES
DYES
ELECTROPHORESIS
EXCITATION
FLUORESCENCE
LASER RADIATION
SEPARATION PROCESSES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
ELECTROMAGNETIC RADIATION
ENERGY-LEVEL TRANSITIONS
LUMINESCENCE
ORGANS
RADIATIONS
SEMICONDUCTOR DEVICES
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry
400105 - Separation Procedures