Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries

doi:https://doi.org/10.1021/ac00081a010

Title: Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries

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Abstract

Various excitation schemes for distributing a laser beam to a large number of capillaries in an array are evaluated. This led to the construction of a multiplexed system for monitoring the electrophoresis of DNA fragments in 100 capillaries. The laser-excited fluorescence signals from each capillary are simultaneously recorded at the rate of 0.6 frame/s by a CCD camera. The reconstructed electropherograms show excellent reproducibility and minimal cross-talk. The system provides for two simultaneous excitation wavelengths so that it can be adapted for two-color, two-intensity DNA sequencing based on the commercial four-dye chemistry. Only 20 mW per laser line was employed. Further development of this system to accommodate up to 4096 independent sequencing channels at a time is discussed. 26 refs., 6 figs., 2 tabs.

Authors:

Ueno, Kyoji; Yeung, E S ^[1]

Ames Lab., IA (United States)

Publication Date:: 1994-05-01

OSTI Identifier:: 7265367

DOE Contract Number:: W-7405-ENG-82

Resource Type:: Journal Article

Journal Name:: Analytical Chemistry (Washington); (United States)

Additional Journal Information:: Journal Volume: 66:9; Journal ID: ISSN 0003-2700

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; DNA SEQUENCING; MONITORING; CAMERAS; CAPILLARIES; CHARGE-COUPLED DEVICES; DYES; ELECTROPHORESIS; EXCITATION; FLUORESCENCE; LASER RADIATION; SEPARATION PROCESSES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; ELECTROMAGNETIC RADIATION; ENERGY-LEVEL TRANSITIONS; LUMINESCENCE; ORGANS; RADIATIONS; SEMICONDUCTOR DEVICES; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics; 550200 - Biochemistry; 400105 - Separation Procedures

Citation Formats


                    Ueno, Kyoji, and Yeung, E S. Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries.  United States: N. p., 1994. 
        Web.  doi:10.1021/ac00081a010.

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                    Ueno, Kyoji, & Yeung, E S. Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries.  United States.  https://doi.org/10.1021/ac00081a010

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                    Ueno, Kyoji, and Yeung, E S. Sun .  
        "Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries".  United States.  https://doi.org/10.1021/ac00081a010.

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@article{osti_7265367,

  title        = {Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries},

  author       = {Ueno, Kyoji and Yeung, E S},

  abstractNote = {Various excitation schemes for distributing a laser beam to a large number of capillaries in an array are evaluated. This led to the construction of a multiplexed system for monitoring the electrophoresis of DNA fragments in 100 capillaries. The laser-excited fluorescence signals from each capillary are simultaneously recorded at the rate of 0.6 frame/s by a CCD camera. The reconstructed electropherograms show excellent reproducibility and minimal cross-talk. The system provides for two simultaneous excitation wavelengths so that it can be adapted for two-color, two-intensity DNA sequencing based on the commercial four-dye chemistry. Only 20 mW per laser line was employed. Further development of this system to accommodate up to 4096 independent sequencing channels at a time is discussed. 26 refs., 6 figs., 2 tabs.},

  doi          = {10.1021/ac00081a010},

  url          = {https://www.osti.gov/biblio/7265367},
  journal      = {Analytical Chemistry (Washington); (United States)},

  issn         = {0003-2700},

  number       = ,

  volume       = 66:9,

  place        = {United States},

  year         = {1994},

  month        = {5}

}

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