Effects of auxiliary carbon sources and electron acceptors on methanogenic degradation of chlorinated phenols

Journal Article · Sun Aug 01 00:00:00 EDT 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620120808· OSTI ID:7264918

Haeggblom, M M; Rivera, M D; Young, L Y ^[1]

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1. New York Univ. Medical Center, New York, NY (United States)

The authors studied the degradation of chlorinated phenols under methanogenic conditions by establishing enrichment cultures on 4-chlorophenol and 2,4-dichlorophenol with or without a supplementary substrate. p-Cresol was chosen as a nonchlorinated aromatic compound structurally similar to the chlorophenols, and propionate was chosen as a readily utilizable carbon source. 2,4-Dichlorophenol was dechlorinated to 4-chlorophenol, which was degraded without further detection of metabolites. The rates of chlorophenols and supplementary substrates. The addition of p-cresol or propionate as an auxiliary carbon source enhanced the rate of 4-chlorophenol degradation. Methanogenic cultures capable of ortho dechlorination were repeatedly subcultured by dilution into fresh media and refeeding of 2,6-dichlorophenol and either p-cresol or propionate as auxiliary substrates. 2,6-Dichlorophenol was sequentially dechlorinated to 2-chlorophenol and phenol and ultimately mineralized to methane and carbon dioxide. Cultures adapted to 2,4- or 2,6-dichlorophenol also readily dechlorinated other dichlorophenols containing an ortho chlorine. The alternative electron acceptors nitrate, sulfite, and thiosulfate completely inhibited dechlorination of 2,6-dichlorophenol, whereas sulfate slowed the dechlorination rate.

• OSTI ID: 7264918
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 12:8; ISSN ETOCDK; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CRESOLS
DECOMPOSITION
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
HYDROXY COMPOUNDS
METABOLISM
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHENOLS