Further characterization of the circulating cell in chronic lymphocytic leukemia
Peripheral lymphocytes from normal individuals and from patients with chronic lymphocytic leukemia (CLL) were cultured in vitro for 1-7 days. The growth response to phytohemagglutinin (PHA) was quantitated by the incorporation of tritiated uridine into RNA nucleotide during a 2-hr pulse with the radioisotope. While the maximum response in PHA-stimulated normal cultures appeared at 2-3 days, CLL cultures required 5-7 days to develop their maximal response, which was 50 percent-60 percent of the normal magnitude. Dilution of the number of normally reactive lymphocytes by culturing them with totally unreactive, mitomycin-treated cells produced a normal 72-hr maximal response, no matter what proportion of unreactive cells was included in the PHA-stimulated cultures. In addition, the response of peripheral lymphocytes from patients with myeloblastic leukemia, where large numbers of unreactive myeloblasts diluted the normal small lymphocytes, a depressed reaction occurred at the anticipated 2-3 days. Nylon fiber-adherent lymphocytes consisting of 85 percent immunoglobulin (Ig)-bearing cells responded minimally to PHA, but showed no evidence of a delay. When isolated from CLL patients, both fiber-adherent cells (ig-bearing) as well as non-fiber-adherent (sheep erythrocyte-rosetting) cells responded to PHA in a delayed fashion. Similarly, a case of CLL, in which 93.5 percent of the circulating lymphocytes bore sheep red blood cell receptors, showed its peak response to PHA at 7 days. Therefore, using surface marker criteria considered characteristic of normal T cells and B cells, the delayed response to PHA on the part of CLL lymphocytes was independent of thymic or nonthymic origin.
- Research Organization:
- Coll. of Medicine and Dentistry of New Jersey, Newark
- OSTI ID:
- 7263580
- Journal Information:
- Blood; (United States), Vol. 48:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
CELL PROLIFERATION
MITOMYCIN
BIOLOGICAL EFFECTS
PHYTOHEMAGGLUTININ
CELL CULTURES
LEUKEMIA
PATIENTS
RNA
TRITIUM
AGGLUTININS
ANIMAL CELLS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
HEMAGGLUTININS
HEMIC DISEASES
HYDROGEN ISOTOPES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MUCOPROTEINS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES
550300* - Cytology