Canine allogeneic bone marrow transplantation: technique and variables influencing engraftment. [Gamma radiation]

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-197608000-00010· OSTI ID:7263482

Bull, M I ^[1]; Herzig, G P; Graw, Jr, R G; Krueger, G R.F.; Bowles, C A

---

1. National Cancer Inst., Bethesda, MD

We have studied the toxicity and immune suppression of supralethal total body irradiation (800-2000 rads, /sup 60/Co) at three dose intensities (10 rads/min, 49 rads/min, and 100 rads/min). In 79 intensively supported radiation control animals, the LD/sub 50(5)/ (that theoretical dose at which 50 percent of the animals will die within 5 days) for these dose intensities is estimated to be 1556, 941, and 921 rads, respectively. A biomodal pattern of early (median 4 days) and late (median 9 days) deaths was observed corresponding to histopathological evidence of the intestinal and hematopoietic radiation syndromes. Random donor bone marrow transplants were performed in 83 animals to test immune suppression afforded by 800 rads and 1000 rads at dose intensities of either 10 rads/min or 49 rads/min. Bone marrow cell dose was varied to analyze its effect on engraftment. A greater degree of immunosuppression with less toxicity was achieved at the lower dose intensity. A minimum dose of 3-5 x 10/sup 8/ nucleated allogeneic bone marrow cells/kg (readily obtainable from living donors) resulted in a high percentage of engraftment with lethal graft-versus-host disease following conditioning with 1,000 rads midplane at 10 rads/min, the optimum regimen employed.

• OSTI ID: 7263482
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 22:2; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DOGS
DOSES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
LETHAL DOSES
LETHAL RADIATION DOSE
MAMMALS
RADIATION DOSES
RADIATION EFFECTS
RADIATION SYNDROME
RADIATIONS
SOMATIC CELLS
TOXICITY
TRANSPLANTS
VERTEBRATES