Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro

Journal Article · Mon Jun 01 00:00:00 EDT 1992 · Biomedical and Environmental Sciences; (United States)

OSTI ID:7262686

Mukherjee, D; Guha, D; Kumar, V ^[1]

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1. Department of Zoology, University of Kalyani (India)

Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.

• OSTI ID: 7262686
• Journal Information: Biomedical and Environmental Sciences; (United States), Vol. 5:2; ISSN 0895-3988
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM
TOXICITY
CHOLESTEROL
BIOSYNTHESIS
FISHES
SENSITIVITY
MERCURY CHLORIDES
OXIDOREDUCTASES
ENZYME ACTIVITY
PHENOL
SULFIDES
GONADOTROPINS
INHIBITION
OOCYTES
OVARIES
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
ELEMENTS
ENZYMES
FEMALE GENITALS
GERM CELLS
GONADS
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
MERCURY COMPOUNDS
MERCURY HALIDES
METALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOLS
PITUITARY HORMONES
POLLUTION
PROTEINS
STEROIDS
STEROLS
SULFUR COMPOUNDS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology