Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro

Journal Article · Mon Jun 01 00:00:00 EDT 1992 · Biomedical and Environmental Sciences; (United States)

OSTI ID:7262686

Mukherjee, D; Guha, D; Kumar, V ^[1]

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1. Department of Zoology, University of Kalyani (India)

Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.

• OSTI ID: 7262686
• Journal Information: Biomedical and Environmental Sciences; (United States), Journal Name: Biomedical and Environmental Sciences; (United States) Vol. 5:2; ISSN 0895-3988; ISSN BESCE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOSYNTHESIS
BODY
CADMIUM
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
CHOLESTEROL
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FEMALE GENITALS
FISHES
GERM CELLS
GONADOTROPINS
GONADS
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INHIBITION
MERCURY CHLORIDES
MERCURY COMPOUNDS
MERCURY HALIDES
METALS
OOCYTES
ORGANIC COMPOUNDS
ORGANS
OVARIES
OXIDOREDUCTASES
PEPTIDE HORMONES
PHENOL
PHENOLS
PITUITARY HORMONES
POLLUTION
PROTEINS
SENSITIVITY
STEROIDS
STEROLS
SULFIDES
SULFUR COMPOUNDS
SYNTHESIS
TOXICITY
VERTEBRATES
WATER POLLUTION