Reduction of fatal graft-versus-host disease by /sup 3/H--thymidine suicide of donor cells cultured with host cells. [Mice, gamma radiation]
The effect of the tritiated thymidine (/sup 3/H-TdR) suicide technique on the ability of donor cells to induce fatal graft-versus-host disease (GVHD) was studied. C57BL/6 (H-2/sup b/) spleen cells were stimulated in vitro with irradiated BALB/c (H-2/sup d/) Moloney lymphoma cells in mixed culture and /sup 3/H-TdR of high-specific activity added to eliminate proliferating cells. The ability of such cells to induce fatal GVHD was assayed by injecting them i.v. into adult BALB/c mice immunosuppressed with cyclophosphamide (180 mg/kg). These cells induced fatal GVHD in fewer mice (52 percent) than did C57BL/6 cells cultured with BALB/c lymphoma cells but without /sup 3/H-TdR (87 percent) and C57BL/6 cells cultured with irradiated C57BL/6 cells with (95 percent) or without /sup 3/H-TdR (86 percent). Thus, the /sup 3/H-TdR suicide technique greatly diminished the ability of cells to induce lethal GVHD.
- Research Organization:
- Univ. of Washington, Seattle
- OSTI ID:
- 7259826
- Journal Information:
- Transplantation; (United States), Vol. 23:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GRAFT-HOST REACTION
CONTROL
SPLEEN CELLS
BIOLOGICAL RADIATION EFFECTS
TUMOR CELLS
IMMUNOSUPPRESSION
CELL CULTURES
CELL PROLIFERATION
GAMMA RADIATION
MICE
THYMIDINE
TRITIUM
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IONIZING RADIATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)