Nature of the repair process associated with the recovery of Escherichia coli after exposure to Cd/sup 2 +/

Journal Article · Mon Feb 21 04:00:00 EST 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)90604-0· OSTI ID:7256540

Mitra, R S; Bernstein, I A

When different strains of Escherichia coli are exposed to Cd/sup 2 +/, the cells accommodate after a long lag and proliferate. The time required for this response depends on the nature of the strain and the supplements in the growth medium. Immediately after exposure to Cd/sup 2 +/, considerable single strand breaks in the DNA are observed but the DNA is repaired prior to the initiation of cell proliferation. The finding that accommodation occurs in DNA polymerase I-deficient mutant cells suggests that DNA polymerase I may not be required for repair of damaged DNA in Cd/sup 2 +/-exposed cells. The recovery of Cd/sup 2 +/-exposed cells in a temperature-sensitive DNA ligase mutant cells at the permissive temperature (30/sup 0/C) and failure to recover at the non-permissive temperature (42/sup 0/C) indicates, however, that DNA ligase is involved in the repair of the single strand breaks associated with Cd/sup 2 +/-induced damage.

• Research Organization: Univ. of Michigan, Ann Arbor
• OSTI ID: 7256540
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 74:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CADMIUM
CELL PROLIFERATION
DNA
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GROWTH
LIGASES
METALS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUTRIENTS
ORGANIC COMPOUNDS
POLYMERASES
RECOVERY
REPAIR
STRAND BREAKS
TEMPERATURE EFFECTS
TIME DEPENDENCE