The kinetics and mechanisms of iron removal from human serum transferrin
Thesis/Dissertation
·
OSTI ID:7255532
Iron removal from transferrin by the synthetic catechol sequestering agent, 1,5,10-N,N{prime},N{double prime} - tris(sulfo-2,3-dihydroxybenzoyl)triazadecane, is investigated at pH 7.4 and a range of temperatures and ionic strengths. Biphasic kinetic behavior is observed for iron removal from diferric transferrin and is attributed to kinetic inequivalence between the two sites. The kinetic data are fit to a model which incorporates this assumption. Elucidation of the microscopic rate constants for ion removal from the individual sites is achieved through studies or iron removal from the monoferric transferrins and differic transferrin in which either site is exclusively labeled with radioactive iron. The kinetics of iron release from the ferric transferrins by several types of ligands are carried out to discriminate between proposed mechanisms. The first spectroelectrochemical measurement of the formal reduction potential of iron transferrin is carried out using methyl viologen to mediate electron transfer to the protein. Electrophoretically purified {sup 57}Fe-enriched monoferric and diferric transferrins are studied by Moessbauer spectroscopy. The electronic spectra of the two sites are described by the same set of spin-Hamiltonian parameters.
- Research Organization:
- California Univ., Berkeley, CA (USA)
- OSTI ID:
- 7255532
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
ELECTRON TRANSFER
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IRON
IRON 57
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGANDS
MAMMALS
MAN
METALLOPROTEINS
METALS
NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
REMOVAL
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENTS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
ELECTRON TRANSFER
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IRON
IRON 57
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGANDS
MAMMALS
MAN
METALLOPROTEINS
METALS
NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
REMOVAL
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENTS
VERTEBRATES