Two-step stimulation of B lymphocytes to enter DNA synthesis: Synergy between anti-immunoglobulin antibody and cytochalasin on expression of c-myc and a G/sub 1/-specific gene

Journal Article · Tue Mar 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.3.1371· OSTI ID:7253647

Buckler, A J; Rothstein, T L; Sonenshein, G E

Previously the authors demonstrated that stimulation of resting murine splenic B lymphocytes with goat anti-mouse immunoglobulin antibody (GaMIg) plus cytochalasin D (CD) led to DNA synthesis; GaMIg and CD added simultaneously, or GaMIg added before CD, induced this response. Cells similarly treated with GaMIg or CD alone did not enter S phase. Here they have measured the effects of this two-signal stimulation on the c-myc, 2F1, and ..gamma..-actin genes. The expression of these growth-related genes is known to change either during the G/sub 0/-to-G/sub 1/ phase of the cell cycle. For the 2F1 and c-myc genes, neither the GaMIg nor CD stimulus alone led to a prolonged increase in mRNA levels, whereas GaMIg plus CD allowed for continuous elevated expression of these genes. Furthermore, GaMIg pretreatment rendered expression of the c-myc and 2F1 genes susceptible to subsequent action by CD. In contrast, CD alone was sufficient to produce changes in ..gamma..-actin gene expression. Thus there are synergistic effects of competence- and progression like factors on the expression of the c-myc and 2F1 genes, and these effects correlate with the progression of B lymphocytes to DNA synthesis.

• Research Organization: Dept. of Microbiology, Boston Univ. School of Medicine, 80 East Concord Street, Boston, MA (US)
• OSTI ID: 7253647
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:3; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
DNA REPLICATION
DOMESTIC ANIMALS
EXPERIMENTAL DATA
FUNCTIONS
GENE RECOMBINATION PROTEINS
GENES
GLOBULINS
GOATS
IMMUNOGLOBULINS
IMMUNOLOGY
INFORMATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
NUMERICAL DATA
ONCOGENES
ORGANIC COMPOUNDS
PHENOTYPE
PROTEINS
RODENTS
RUMINANTS
SOMATIC CELLS
STIMULATION
SYNTHESIS
VERTEBRATES