Imaging biological specimens with high-intensity soft x rays

Journal Article · Fri Oct 31 23:00:00 EST 1986 · J. Opt. Soc. Am. B: Opt. Phys.; (United States)

DOI:https://doi.org/10.1364/JOSAB.3.001551· OSTI ID:7249095

Solem, J C

I compare alternative techniques for x-ray imaging of biological specimens on the basis of (1) transverse and longitudinal resolution, (2) depth of field, (3) choice of recording medium, and (4) recording efficiency. I show that for all imaging techniques, the dosages suffered by specimens are so high that the living state cannot be preserved, nor can the structural integrity of the specimen be maintained in the usual sense. I demonstrate the remarkable fact that, at sufficiently high intensity, an image of diffraction-limited resolution can be captured before the specimen is obliterated.

• Research Organization: Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
• OSTI ID: 7249095
• Journal Information: J. Opt. Soc. Am. B: Opt. Phys.; (United States), Journal Name: J. Opt. Soc. Am. B: Opt. Phys.; (United States) Vol. 3:11; ISSN JOBPD
• Country of Publication: United States
• Language: English

Related Subjects

440300* -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL MATERIALS
DOSES
EFFICIENCY
ELECTROMAGNETIC RADIATION
EQUIPMENT
EXTREME ULTRAVIOLET RADIATION
HOLOGRAPHY
LASL
MATERIALS
MICROSCOPES
MICROSCOPY
NATIONAL ORGANIZATIONS
PLANT CELLS
RADIATION DOSES
RADIATIONS
RESOLUTION
ULTRAVIOLET RADIATION
US AEC
US DOE
US ERDA
US ORGANIZATIONS
X-RAY EQUIPMENT