Glycosphingolipid biosynthesis in adult mouse kidney cultures

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7246765

Lyerla, T A; Gross, S K; McCluer, R H

Primary kidney cultures from C57BL/6J mice, six weeks of age or older, were produced using D-valine medium to select for epithelial cell growth. After allowing cells to attach and proliferate for one week following plating, medium was changed once per week. Cells formed nearly confluent monolayers during the second week of culture. The cultured cells contained all of the glycosphingolipids seen in the adult kidney, as analyzed by HPLC as the perbenzoyl derivatives. Glucosylceramide, however, was highly predominant in the cultured cells, whereas dihexosyl- and trihexosylceramides predominate in the intact kidney. Sex differences in glycolipid contents found in the intact kidney were also apparent in these cultured cells: greater amounts of nonhydroxy fatty acid (Nfa) containing galactosylceramide and Nfa digalactosylceramide were present in the male cells. Neutral glycosphingolipids were labeled after two weeks in culture using /sup 3/H-labeled palmitate. The /sup 3/H-palmitate was incorporated into all of the glycolipids within two hours of labeling, and after three days of chase with unlabeled palmitate, some redistribution into higher glycosphingolipids was apparent. Hence, adult mouse kidney cells in D-valine medium retain their differentiated characteristics for a sufficient period of time to allow investigation of glycolipid syntheses in monolayer cultures of epithelial cells derived from this organ.

• Research Organization: Clark Univ., Worcester, MA
• OSTI ID: 7246765
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANIMAL TISSUES
ANIMALS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CULTURES
CELL PROLIFERATION
CHROMATOGRAPHY
EPITHELIUM
GLYCOLIPIDS
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LABELLING
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MICE
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SEX DEPENDENCE
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES