Purification and properties of thioether methyltransferase
Thesis/Dissertation
·
OSTI ID:7245891
A method to assay activity was developed which measures acceptance of methyl groups from (methyl-{sup 3}H)-S-adenosylmethionine by dimethyl selenide. The product, ({sup 3}H)trimethylselenonium ion, is separated by HPLC and quantitated by scintillation counting. Thioether methyltransferase from mouse liver and lung resides primarily in the cytosol. In terms of specific activity the enzyme is most active in the lung and liver. Purification from lung cytosol requires a three-step process of DEAE and gel filtration column chromatographies followed by chromatofocusing. SDS-Polyacrylamide gel electrophoresis shows a single homogeneous band with a molecular mass of 28,000 daltons. Vmax and Km values for dimethyl selenide as a substrate are 15. 7 pmol/min and 0.44 {mu}M, respectively. Our studies have also shown that this purified enzyme is capable of methylating a wide range of compounds. To further test the enzyme's role in detoxification, in vivo studies were performed by injecting mice with substrate and (methyl-{sup 3}H)methionine and analyzing tissue extracts and urine for (methyl-{sup 3}H)sulfonium.
- Research Organization:
- Louisville Univ., KY (USA)
- OSTI ID:
- 7245891
- Country of Publication:
- United States
- Language:
- English
Similar Records
Purification and properties of S-adenosylmethionine:dimethylselenide methyltransferase from the mouse
Biosynthesis and urinary excretion of methyl sulfonium derivatives of the sulfur mustard analog, 2-chloroethyl ethyl sulfide, and other thioethers
Microbial transformations of selenium
Conference
·
Fri May 01 00:00:00 EDT 1987
· Federation Proceedings, Federation of American Societies for Experimental Biology; (USA)
·
OSTI ID:5036247
Biosynthesis and urinary excretion of methyl sulfonium derivatives of the sulfur mustard analog, 2-chloroethyl ethyl sulfide, and other thioethers
Journal Article
·
Fri Nov 30 23:00:00 EST 1990
· FASEB Journal (Federation of American Societies for Experimental Biology); (USA)
·
OSTI ID:5707504
Microbial transformations of selenium
Journal Article
·
Fri Dec 31 23:00:00 EST 1976
· Appl. Environ. Microbiol.; (United States)
·
OSTI ID:6300969
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMICAL PROPERTIES
CHROMATOGRAPHY
COUNTING TECHNIQUES
DETOXIFICATION
DIGESTIVE SYSTEM
DRUGS
ELECTROPHORESIS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
MAMMALS
METHIONINE
METHYL TRANSFERASES
MICE
MOLECULAR WEIGHT
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SCINTILLATION COUNTING
SELENIUM
SEMIMETALS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMICAL PROPERTIES
CHROMATOGRAPHY
COUNTING TECHNIQUES
DETOXIFICATION
DIGESTIVE SYSTEM
DRUGS
ELECTROPHORESIS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
MAMMALS
METHIONINE
METHYL TRANSFERASES
MICE
MOLECULAR WEIGHT
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SCINTILLATION COUNTING
SELENIUM
SEMIMETALS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES