Repair of cis-platinum-DNA adducts by ABC excinuclease in vivo and in vitro

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · J. Bacteriol.; (United States)

OSTI ID:7245078

Husain, I; Chaney, S G; Sancar, A

cis-Platinum compounds, which are used in cancer chemotherapy, are thought to exert their effect by damaging DNA. It is known that this damage is partially repaired in Escherichia coli. Using cis-Pt-treated pBR322 DNA as a probe, the role of nucleotide excision repair in the removal of Pt-DNA adducts was investigated. It was found that the nucleotide excision pathway was the major mechanism for repairing Pt adducts in transforming plasmid DNA but that a recA-dependent pathway also contributed to plasmid survival. When cis-Pt-damaged pBR322 was treated with the purified nucleotide excision enzyme ABC excinuclease in vitro, a fraction of the adducts was removed by the enzyme; this removal resulted in a corresponding increase in transformation efficiency.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 7245078
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 163:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANTINEOPLASTIC DRUGS
BACTERIA
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA ADDUCTS
DRUGS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
HYDROLASES
IN VITRO
IN VIVO
MICROORGANISMS
NUCLEASES
PHOSPHODIESTERASES
PLASMIDS
PLATINUM COMPOUNDS
RECOVERY
REPAIR
TOXICITY
TRANSITION ELEMENT COMPOUNDS