Determination of the stability of dopamine in aqueous solutions by high performance liquid chromatography

Journal Article · Fri Dec 31 23:00:00 EST 1993 · Journal of Liquid Chromatography; (United States)

DOI:https://doi.org/10.1080/10826079408013178· OSTI ID:7242234

Shen, Y ^[1]; Ye, M Y ^[2]

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1. Purdue Univ., West Lafayette, IN (United States). Dept. of Veterinary Physiology and Pharmacology
2. Pacific Northwest Lab., Richland, WA (United States). Dept. of Biology and Chemistry ManTech Environmental Technology, Inc., Ada, OK (United States)

Methods for the analysis of dopamine and its degradation products in aqueous solutions are described. The technique of reverse phase chromatography with electrochemical detection is used to investigate the stability of dopamine in various aqueous solutions. In neutral and basic solutions, dopamine is rapidly oxidized by dissolved oxygen to form degradation products. The results demonstrate that dopamine is stable in 0.1 N HCl solution, pH < 1. The study indicates that EDTA can slow down the oxidation process. The detection limit for the analysis of dopamine is 0.1 [mu]M with 100 [mu]l injection.

• OSTI ID: 7242234
• Journal Information: Journal of Liquid Chromatography; (United States), Journal Name: Journal of Liquid Chromatography; (United States) Vol. 17:7; ISSN 0148-3919; ISSN JLCHD8
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism &amp; Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHELATING AGENTS
CHROMATOGRAPHY
DOPAMINE
DRUGS
EDTA
HYDROXY COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
SEPARATION PROCESSES
STABILITY
SYMPATHOMIMETICS