Ultraviolet irradiation of nucleic acids: formation, purification, and solution conformational analyses of the Cis-Syn and trans-syn photodimers of UpU

Journal Article · Tue May 03 04:00:00 EDT 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00409a005· OSTI ID:7240803

Rycyna, R E; Alderfer, J L

Uridylyl(3'-5')uridine (UpU) is subjected to aqueous acetone photosensitized radiation with sunlamps. These irradiation conditions form only cyclobutane-type photodimers. Purification of a specific configurational photodimer is accomplished by using C-18 reverse-phase high-performance liquid chromatography. Multinuclear NMR analysis is used to analyze photoproduct formation and to determine conformational features of these photodimers. Four photodimers are identified, with the cis-syn isomer predominant. The cis-syn and trans-syn photodimers of UpU exhibit markedly different furanose and exocyclic bond conformations. A comparison of the properties of the cis-syn dimers of UpU with those of dTpdT reveal many similar conformational features but also some that are different.

• Research Organization: Roswell Park Memorial Institute, Buffalo, NY (USA)
• OSTI ID: 7240803
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:9; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AQUEOUS SOLUTIONS
AZINES
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMATOGRAPHY
CONFORMATIONAL CHANGES
DISPERSIONS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
FAR ULTRAVIOLET RADIATION
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAGNETIC RESONANCE
MIXTURES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
PYRIMIDINE DIMERS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RESONANCE
RIBOSIDES
SEPARATION PROCESSES
SOLUTIONS
STABLE ISOTOPES
ULTRAVIOLET RADIATION
URACILS
URIDINE