Simple determination of the CO sub 2 /O sub 2 specificity of Ribulose-1,5-bisphosphate carboxylase/oxygenase by the specific radioactivity of ( sup 14 C) glycerate 3-phosphate
Journal Article
·
· Plant Physiology; (United States)
OSTI ID:7236274
- Univ. of Arizona, Tucson (United States)
A new method is presented for measurement of the CO{sub 2}/O{sub 2} specificity factor of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). The ({sup 14}C)3-phosphoglycerate (PGA) from the Rubisco carboxylase reaction and its dilution by the Rubisco oxygenase reaction was monitored by directly measuring the specific radioactivity of PGA. {sup 14}CO{sub 2} fixation with Rubisco occurred under two reaction conditions: carboxylase with oxygenase with 40 micromolar CO{sub 2} in O{sub 2}-saturated water and carboxylase only with 160 micromolar CO{sub 2} under N{sub 2}. Detection of the specific radioactivity used the amount of PGA as obtained from the peak area, which was determined by pulsed amperometry following separation by high-performance anion exchange chromatography and the radioactive counts of the ({sup 14}C)PGA in the same peak. The specificity factor of Rubisco from spinach (Spinacia oleracea L.) (93 {plus minus} 4), from the green alga Chlamydomonas reinhardtii (66 {plus minus} 1), and from the photosynthetic bacterium Rhodospirillum rubrum (13) were comparable with the published values measured by different methods.
- OSTI ID:
- 7236274
- Journal Information:
- Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 98:2; ISSN 0032-0889; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
Mon Mar 31 23:00:00 EST 1986
· Plant Physiol.; (United States)
·
OSTI ID:7025030
Status of the substrate binding sites of ribulose bisphosphate carboxylase as determined with 2-C-carboxyarabinitol 1,5-bisphosphate. [Spinacia oleracea]
Journal Article
·
Tue May 01 00:00:00 EDT 1990
· Plant Physiology; (USA)
·
OSTI ID:5924472
Proton transfer reactions of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase
Conference
·
Mon Dec 30 23:00:00 EST 1996
·
OSTI ID:538944
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLATION
CHALCOGENIDES
CHEMICAL REACTIONS
CHLAMYDOMONAS
CHLOROPHYCOTA
ELEMENTS
ENZYMES
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PHOTOSYNTHETIC BACTERIA
PLANTS
PROTEINS
RADIOACTIVITY
RIBULOSE DIPHOSPHATE CARBOXYLASE
SPECIFICITY
SPINACH
TRACER TECHNIQUES
UNICELLULAR ALGAE
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLATION
CHALCOGENIDES
CHEMICAL REACTIONS
CHLAMYDOMONAS
CHLOROPHYCOTA
ELEMENTS
ENZYMES
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PHOTOSYNTHETIC BACTERIA
PLANTS
PROTEINS
RADIOACTIVITY
RIBULOSE DIPHOSPHATE CARBOXYLASE
SPECIFICITY
SPINACH
TRACER TECHNIQUES
UNICELLULAR ALGAE
VEGETABLES