Brain cholinergic involvement in the diurnal variations of the rapid development of tolerance to the hypothermic effect of ethanol
- Florida A and M Univ., Tallahassee (United States)
Male Sprague-Dawley rats maintained under controlled environmental conditions were used. Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities were determined in the hypothalamus, pons, medulla oblongata, thalamus, midbrain, cerebral cortex, cerebellum and hippocampus of control rats and rats treated with ethanol either after a single dose at 10:00 and 22:00, or after a second dose administered 24hrs later at the same time schedules. Results of this experiment indicate that repeated administration with ethanol was associated with rapid development of tolerance to the hypothermic action of ethanol. A single injection of ethanol at 10:00 resulted in significant increase in ChAT activity of pons and cerebellum and decline of ChAT activity of midbrain. There were no significant changes in AChE activity at all of these different brain regions. A single injection of ethanol at 22:00 resulted in significant decrease in ChAT activity of the hypothalamus, pons, midbrain, hippocampus. At the same time, there was a significant decline of AChE activity of the pons, medulla and midbrain. These findings indicate that changes in the responsiveness of the brain cholinergic enzymes may explain the increase in hypothermia and the rapid development of tolerance.
- OSTI ID:
- 7235737
- Report Number(s):
- CONF-9204141--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 6:4; ISSN 0892-6638; ISSN FAJOE
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BODY TEMPERATURE
BRAIN
CARBOXYLESTERASES
CENTRAL NERVOUS SYSTEM
CHOLINE
CHOLINESTERASE
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ETHANOL
HYDROLASES
HYDROXY COMPOUNDS
HYPOTHERMIA
LIPOTROPIC FACTORS
MAMMALS
METABOLISM
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
QUATERNARY COMPOUNDS
RATS
RODENTS
TRANSFERASES
VERTEBRATES