Use of an optical technique to evaluate the cerebral vascular effects of alcohol (A): Effects on deoxyhemoglobin (DH) and levels of reduced cytochrome oxidase (rCO)

Conference · Wed Feb 26 00:00:00 EST 1992 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:7235729

Barbour, R L; Gebiewold, A; Altura, B M ^[1]

---

1. Southern Univ. of New York, Brooklyn (United States)

The dose-response effects of acute A infusion were studied to examine the suggestion that A can induce stroke-like events as a consequence of cerebral vasospasm. By employing a single sending and receiving fiber, an optical backscatter measurement was employed to monitor the levels in DH and rCO in a closed cranium preparation. Anesthetized rats were prepared by cannulating a branch of the internal carotid artery and subjected to either a bolus infusion (BI) or to a constant infusion (CI) of 5 or 10% A at various rates. Results showed that low BI doses of A typically produced a slight increase in the oxyhemoglobin signal indicating that vasodilation had probably occurred. Higher BI doses, however, produced a prompt and significant reduction in the hemoglobin signal with a rise in rCO suggesting a vasoconstrictor response leading to ischemia, followed by recovery within 3-5 min. CI of A produced a similar cerebral vascular response, in a dose-related manner, but of a more sustained nature. At 30-50% of the BI dose levels, a global blanching of the brain surface occurred; rCO levels increased by 50-90% with a corresponding decline in levels of oxyhemoglobin. Control experiments using identical volumes/flow rates of Ringers solution failed to produce any alterations in the optical spectrum. Overall, these data indicate that, depending on dose, (a) A can induce vasodilatory or vasoconstrictor effects in the intact brain; (b) the more pronounced effects involve vasospasm in the cortical microcirculation leading to global ischemia as determined by elevated levels of rCO and DH; (c) optical measurements permit direct noninvasive assessment of the cerebral vascular effects of substances of abuse.

• OSTI ID: 7235729
• Report Number(s): CONF-9204141-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 6:4; Conference: Federation of American Societies for Experimental Biology (FASEB) meeting, Anaheim, CA (United States), 5-9 Apr 1992; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CEREBRAL CORTEX
VASODILATION
CYTOCHROME OXIDASE
ENZYME ACTIVITY
ETHANOL
BIOLOGICAL EFFECTS
HEMOGLOBIN
BIOSYNTHESIS
DOSE-RESPONSE RELATIONSHIPS
ISCHEMIA
METABOLISM
ALCOHOLS
ANEMIAS
BODY
BRAIN
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CEREBRUM
DISEASES
ENZYMES
GLOBINS
HAEM DEHYDROGENASES
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
PORPHYRINS
PROTEINS
SYMPTOMS
SYNTHESIS
VASCULAR DISEASES
560300* - Chemicals Metabolism & Toxicology