Effect of repeated administration of butyrylcholinesterase on antibody induction of rabbits

Technical Report · Thu May 13 04:00:00 EDT 1993

OSTI ID:7234920

Gentry, M K; Nuwayser, E S; Doctor, B P

Protection against toxicity of high doses of organophosphorus compounds (OPs) has been afforded to animals by injection of cholinesterases, with exogenous enzyme acting as a scavenger for the OPs. Implications of repeated doses of enzyme, such as might be needed to counteract effects of multiple exposures to OPs, have not been determined. The effect of repeated intramuscular injections of horse serum butyrylcholinesterase (BChE) was examined in rabbits by monitoring blood enzyme and anti-enzyme immunoglobulin G (IgG) levels. Blood BChE levels were elevated in proportion to the amount of enzyme injected, and enzyme concentrations rose until the production of anti-enzyme antibody occurred. When antibody levels fell to low preinjection levels, at approximately eight weeks, animals were re-injected with a single bolus of enzyme. Most of the rabbits, upon reinjection, exhibited significant elevations in blood enzyme levels, which again decreased as IgG concentrations increased. After antibody levels again decreased to a low, constant value, another bolus of enzyme was administered at 21 weeks. Although antibodies induced by the injections of BChE cleared the exogenously administered BChE, they did not cause adverse reactions, and animals could tolerate the repeated doses of enzyme.

• Research Organization: Walter Reed Army Inst. of Research, Washington, DC (United States). Div. of Biochemistry
• OSTI ID: 7234920
• Report Number(s): AD-P-008859/1/XAB; CNN: DAMD17-92-C-2047
• Country of Publication: United States
• Language: English

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