Methylcobalamin: methylation of platinum and demethylation with lead

Journal Article · Thu Jan 01 04:00:00 EST 1976 · J. Environ. Sci. Health, Part A; (United States)

DOI:https://doi.org/10.1080/10934527609385766· OSTI ID:7234605

Taylor, R T; Hanna, M L

Aerobic incubation of ..mu..M levels of K/sub 2/PtCl/sub 6/ and methylcobalamin (MeB-12) at pH 2.0 (0.01 M HCl) results in the complete conversion of MeB-12 to aquoB-12 without the accumulation of any corrinoid intermediates. Platinic sulfate (Pt(SO/sub 4/)/sub 2/) is also reactive; whereas, K/sub 2/PtCl/sub 4/ alone is unreactive. Soluble lead salts containing Pb/sup 2 +/ were likewise inactive; however, partial to complete demethylation was observed by prolonged incubation with fine suspensions of Pb/sup 4 +/ compounds. Demethylation of (Me-/sup 14/C)MeB-12 with Pb/sup 4 +/ oxides was accompanied by a proportionate volatilization of the label and only unreacted radioactive MeB-12 was detected upon paper electrophoresis. In contrast, demethylation with K/sub 2/PtCl/sub 6/ occurs with a high recovery of nonvolatile /sup 14/C which migrates with a major zone of Pt upon electrophoresis. There is no selective loss of /sup 3/H when (Me-/sup 3/H)MeB-12 reacts with K/sub 2/PtCl/sub 6/. The Me-Pt product has been isolated and characterized with respect to its light absorption spectrum in aqueous solutions.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 7234605
• Journal Information: J. Environ. Sci. Health, Part A; (United States), Journal Name: J. Environ. Sci. Health, Part A; (United States) Vol. 11:3; ISSN JESED
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
AEROBIC CONDITIONS
AIR POLLUTION
AUTOMOBILES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBON 14
CARBON ISOTOPES
ELECTROPHORESIS
ELEMENTS
ENVIRONMENTAL EFFECTS
EVEN-EVEN NUCLEI
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROGEN ISOTOPES
IODINATED ALIPHATIC HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEAD
LIGHT NUCLEI
METALS
METHYL IODIDE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PLATINUM
PLATINUM METALS
POLLUTION
RADIOISOTOPES
REACTION KINETICS
SPECTRA
TRACER TECHNIQUES
TRANSITION ELEMENTS
TRITIUM
VEHICLES
YEARS LIVING RADIOISOTOPES