Measurement of cerebral blood flow in the rat

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Stroke; (United States)

DOI:https://doi.org/10.1161/01.STR.6.6.630· OSTI ID:7226337

Matsumoto, A; Namon, R; Utsunomiya, Y; Kogure, K; Scheinberg, P; Reinmuth, O M

Cerebral blood flow (CBF) was determined in the rat under 70 percent nitrous oxide anesthesia and pentobarbital anesthesia. The application of the Fick principle technique of Kety et al. was modified utilizing /sup 133/Xe infused intravenously steadily for 30 seconds, at which time the animal was decapitated and the head frozen in liquid nitrogen. A prior femoral artery to femoral vein shunt was led through a polyethylene catheter of 0.13 ml volume. This catheter passed as a coil in a NaI crystal well-counter with the arterial /sup 133/Xe concentration curve recorded by a ratemeter-recorder system. The results of the hemispheric blood flow (HBF) were under 70 percent nitrous oxide anethesia in normocapnia (Paco/sub 2/ 38 mm Hg), 86 +- 15 ml/100 gm per minute; with hypocapnia (Paco/sub 2/ 20 mm Hg), 40 +- 5 ml/100 gm per minute; with hypercapnia (Paco/sub 2/ 63 mm Hg), 187 +- 10 ml/100 gm per minute; and with pentobarbital anesthesia (Paco/sub 2/ 38 mm Hg), 41 +- 8 ml/100 gm per minute.

• Research Organization: Univ. of Miami, FL
• OSTI ID: 7226337
• Journal Information: Stroke; (United States), Journal Name: Stroke; (United States) Vol. 6:6; ISSN SJCCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FLOW
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MEASURING METHODS
NERVOUS SYSTEM
NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
VERTEBRATES
XENON 133
XENON ISOTOPES