Extraction and determination of selected polycyclic aromatic hydrocarbons in plant tissues

Journal Article · · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:7225068

Coates, J T; Elzerman, A W; Garrison, A W

A procedure has been developed for analysis of plant material for plycyclic aromatic hydrocarbons (PAHs). Sonication is used to extract the PAHs from homogenized plant material into acetonitrile and then the PAHs are partitioned into pentane. The pentane extract is fractionated on a micro silicic acid column and is analyzed by capillary gas chromatography with flame ionization detection. Overall method recoveries for 16 PAHs spiked into hybrid grain sorghum ranged from 45 to 90%, with relative standard deviations ranging from 3 to 38% and averaging 17%. This method can be used to measured PAHs in hybrid grain sorghum, fescue grass, and similar plants at the 25 ..mu..g/kg level. The procedure was applied to a preliminary study of PAH uptake by sorghum and fescue irrigated with secondary treated municipal wastewater spiked with 5 PAHs. Accumulation of fluoranthene and pyrene by fescue and hybrid grain sorghum from the PAH contaminated irrigation water was apparent.

• Research Organization: Clemson Univ., SC
• OSTI ID: 7225068
• Journal Information: J. Assoc. Off. Anal. Chem.; (United States), Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Vol. 69:1; ISSN JANCA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETONITRILE
ALKANES
AROMATICS
BIOLOGICAL ACCUMULATION
CEREALS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
EXTRACTION
GAS CHROMATOGRAPHY
GRASS
HYDROCARBONS
HYDROGEN COMPOUNDS
IRRIGATION
LIQUID WASTES
NITRILES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PENTANE
PLANTS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRENE
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
SEEDS
SEPARATION PROCESSES
SOLVENT EXTRACTION
UPTAKE
WASTE WATER
WASTES
WATER