Laboratory apparatus for preparing and dispensing nitrogen-15-labeled anhydrous ammonia
An apparatus and a procedure are described for preparing liquid anhydrous NH/sub 3/ (LAA) of a desired /sup 15/N concentration and a cold-jacketed syringe for dispensing the LAA. Preparation of LAA was accomplished by reacting /sup 15/N-labeled (NH/sub 4/)/sub 2/SO/sub 4/ (30-50 atom % /sup 15/N) with a 2 M NaOH solution and allowing the resultant /sup 15/N-labeled NH/sub 3/ gas to mix with reagent grade anhydrous NH/sub 3/ gas dispensed from a lecture bottle (38.5- by 5.1-cm cylinder). The resulting gas mixture was condensed and drained into a collection vessel from which it could be withdrawn, measured, and dispensed with specially built cold-jacketed syringes. With the apparatus and procedure described here, 10 mL of LAA containing 0.7 to 2.0 atom % /sup 15/N can be prepared and applied quantitatively in amounts ranging from 25 (15.21 mg NH/sub 3/-N) to 500 ..mu..L (296.98 mg NH/sub 3/-N). A detailed description of how to construct and operate the /sup 15/N-labeled LAA preparation apparatus and dispensing syringes are discussed.
- Research Organization:
- Univ. of Illinois, Urbana
- OSTI ID:
- 7224347
- Journal Information:
- Soil Sci. Soc. Am. J.; (United States), Journal Name: Soil Sci. Soc. Am. J.; (United States) Vol. 50:1; ISSN SSSJD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
ALKALI METAL COMPOUNDS
AMMONIA
AMMONIUM COMPOUNDS
AMMONIUM SULFATES
CHEMICAL REACTIONS
DESIGN
EQUIPMENT
FABRICATION
HYDRIDES
HYDROGEN COMPOUNDS
HYDROXIDES
ISOTOPES
LABORATORY EQUIPMENT
LIGHT NUCLEI
NITROGEN 15
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PERFORMANCE TESTING
SODIUM COMPOUNDS
SODIUM HYDROXIDES
STABLE ISOTOPES
SULFATES
SULFUR COMPOUNDS
SYNTHESIS
TESTING