Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1

Journal Article · Thu Oct 01 00:00:00 EDT 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7224317

Brand, J M; Cruden, D L; Zylstra, G J; Gibson, D T ^[1]

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1. Univ. of Iowa, Iowa City (United States)

Escheria coli JM109(pDTG601), containing the todC1C2BA genes encoding toluene dioxygenase from Pseudomonas putida F1, oxides indan to (-)-(1R)-indanol (83{percent} R) and trans-1,3-indandiol. Under similar conditions, P.putida F39/D oxidizes indan to (-)-(1R)-indanol (96{percent}R), 1-indanone, and trans-1,3-indandiol. The differences in the enantiomeric composition of the 1-indanols formed by the two organisms are due to the presence of a 1-indanol dehydrogenase in P.putida F39/D that preferentially oxidizes (+)-(1S)-indanol.

• OSTI ID: 7224317
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:10; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIODEGRADATION
BIOTECHNOLOGY
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA
DNA HYBRIDIZATION
DNA-CLONING
ENZYMES
ESCHERICHIA COLI
GENETIC ENGINEERING
HYBRIDIZATION
HYDROCARBONS
HYDROXYLATION
INDAN
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PROTEINS
PSEUDOMONAS
RECOMBINANT DNA
STEREOCHEMISTRY
TOLUENE