Normal and sublethally irradiated stem and granulocyte progenitor cell regeneration in an in vivo culture system. The cellular response to humoral factors released through the action of cyclophosphamide. [Mice, gamma radiation]

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574720· OSTI ID:7221786

MacVittie, T

The in vivo diffusion chamber (DC) method of marrow culture was used to determine if the injection of host mice with cyclophosphamide (CY) caused, through its cytoxic action, the release of a humoral factor(s) capable of initiating stem cell (CFU-s) and granulocyte-macrophage progenitor cell (CFU-c) proliferation. Host mice were injected with CY 1-4 days prior to 800 rad of /sup 60/Co WBI and implantation of DCs containing normal or 400 rad sublethally irradiated (SLI) marrow cells. The greatest proliferative response within CFU-s and CFU-c populations occurred in those mice injected with CY 3 days prior to implant. The marked CFU-s and CFU-c regeneration was initiated during the initial 24 hr of culture in both normal and SLI marrow cells. Thereafter growth rates were approximately the same. SLI marrow, however, showed a greater response to the humoral effects of CY injection than did normal marrow. These data provided evidence that CY induced the release of a diffusible factor(s) capable of accelerating regeneration of normal and sublethally irradiated CFU-s and CFU-c, the magnitude of which was dependent upon the time elapsed between CY injected and implantation of DCs. The marked proliferative response of the SLI stem and progenitor cells to the humoral stimulation may be indicative of the heterogeneity of both CFU-s and CFU-c populations surviving sublethal radiation exposure. The target cells may have possessed a differential sensitivity to the factor(s) initiating cell proliferation.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD
• OSTI ID: 7221786
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
GAMMA RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SOMATIC CELLS
STEM CELLS
VERTEBRATES