In vitro generation of a chemically reactive metabolite of 2,5,2',5'-tetrachlorobiphenyl by rhesus monkey liver microsomes

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-152-39454· OSTI ID:7221663

Seymour, J L; Schmidt, S P; Allen, J R

Incubation of tritiated 2,5,-2,5'-tetrachlorobiphenyl with normal monkey liver microsomes in a NADPH-generating system results in the formation of active metabolite(s) of the (/sup 3/H)2,5,2',5'-tetrachlorobiphenyl capable of covalently binding to RNA and protein isolated from the incubation mixture. The metabolite is not formed when the control microsomes are held at 100/sup 0/ for 10 min prior to incubation. The addition of microsomal supernate to the solution causes an increase in the binding of the active metabolite to macromolecules while the addition of glutathione to the incubation medium significantly inhibits this increase.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 7221663
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 152; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIVER
MACACUS
MAMMALS
METABOLISM
MICROSOMES
MONKEYS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES