Influence of pH upon the Warburg effect in isolated intact spinach chloroplasts. I. Carbon dioxide photoassimilation and glycolate synthesis
Journal Article
·
· Plant Physiol.; (United States)
The influence of pH upon the O/sub 2/ inhibition of /sup 14/CO/sub 2/ photoassimilation (Warburg effect) was examined in intact spinach (Spinacia oleracea) chloroplasts. With conditions which favored the Warburg effect, i.e. rate-limiting CO/sub 2/ and 100 percent O/sub 2/, O/sub 2/ inhibition was greater at pH 8.4 to 8.5 than at pH 7.5 to 7.8. At pH 8.5, as compared with 7.8, there was an enhanced /sup 14/C-labeling of glycolate, and a decrease of isotope in some phosphorylated Calvin cycle intermediates, particularly triose-phosphate. The /sup 14/C-labeling of starch was also more inhibited by O/sub 2/ at higher pH. The enhanced synthesis of glycolate during /sup 14/CO/sub 2/ assimilation at higher pH resulted in a diminution in the level of phosphorylated intermediates of the Calvin cycle, and this was apparently a causal factor of the increased severity of the Warburg effect. The /sup 14/C-labeling profiles have been interpreted in terms of a ''CO/sub 2/''-sensitive as well as a ''CO/sub 2/''-insensitive mechanism for glycolate synthesis. Both mechanisms functioned optimally at the higher pH and both responded to O/sub 2/.
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 7212927
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 59:4; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CHLOROPLASTS
CRYOGENIC FLUIDS
ELEMENTS
EVEN-EVEN NUCLEI
FLUIDS
FOOD
INHIBITION
ISOTOPES
LIGHT NUCLEI
NONMETALS
NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PH VALUE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
POLYSACCHARIDES
RADIOISOTOPES
REAGENTS
SACCHARIDES
SPINACH
STARCH
SYNTHESIS
VEGETABLES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CHLOROPLASTS
CRYOGENIC FLUIDS
ELEMENTS
EVEN-EVEN NUCLEI
FLUIDS
FOOD
INHIBITION
ISOTOPES
LIGHT NUCLEI
NONMETALS
NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PH VALUE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
POLYSACCHARIDES
RADIOISOTOPES
REAGENTS
SACCHARIDES
SPINACH
STARCH
SYNTHESIS
VEGETABLES
YEARS LIVING RADIOISOTOPES