Luteolytic interaction between estradiol and prostaglandin F/sub 2/. cap alpha. in hysterectomized ewes

Journal Article · Wed Jun 01 04:00:00 EDT 1977 · Biol. Reprod.; (United States)

DOI:https://doi.org/10.1095/biolreprod16.5.571· OSTI ID:7208528

Gengenbach, D R; Hixon, J E; Hansel, W

The hypothesis that the luteolytic interaction between prostaglandin F/sub 2/..cap alpha..(PGF/sub 2/..cap alpha..) and estradiol is independent of the uterus was evaluated in hysterectomized and hysterectomized, ovarian x-irradiated ewes. Hysterectomies and either sham or x irradiations were performed 7 or 8 days after observation of estrus. After a 72 h recovery period the effects of injections on two consecutive days of: (1) the vehicles for PGF/sub 2/..cap alpha.. and estradiol benzoate (E/sub 2/B), (2) 3.5 mg PGF/sub 2/..cap alpha.., (3) 750 ..mu..g E/sub 2/B, and (4) 3.5 mg PGF/sub 2/..cap alpha.. + 750 ..mu..g E/sub 2/B were compared in sham and ovarian x-irradiated ewes. Results of plasma progesterone determinations and mean corpus luteum weights showed that treatment with PGF/sub 2/..cap alpha.. alone was not luteolytic. Treatment with E/sub 2/B alone had marginal effects on corpus luteum function. For example, plasma progesterone concentrations were depressed (P < 0.05) when compared to vehicle treated controls at isolated times between 60 and 96 h after the first injection in both sham and x-irradiated ewes, and the mean corpus luteum weight for the x-irradiated group was less than that found for the control groups (P < 0.05). However, following treatment with both PGF/sub 2/..cap alpha.. and E/sub 2/B, complete luteolysis was observed in three of four sham and three of four x-irradiated ewes. These data suggest a luteolytic interaction between estradiol and PGF/sub 2/..cap alpha.. that is independent of the uterus. In a second experiment, hysterectomized sham and x-irradiated ewes were treated with 7.0 mg PGF/sub 2/..cap alpha... Plasma progesterone determinations and corpus luteum weights suggested that complete luteolysis was observed in all sham-irradiated ewes and in two of four x-irradiated ewes. This dose of PGF/sub 2/..cap alpha.. also proved to be effective in three of four x-irradiated, uterine intact ewes. These results suggest that ovarian follicles are not an absolute requirement for PGF/sub 2/..cap alpha..-induced luteolysis.

• Research Organization: Cornell Univ., Ithaca, NY
• OSTI ID: 7208528
• Journal Information: Biol. Reprod.; (United States), Journal Name: Biol. Reprod.; (United States) Vol. 16:5; ISSN BIREB
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CHEMISTRY
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ESTRADIOL
ESTRANES
ESTROGENS
ESTROUS CYCLE
EVALUATION
FEMALE GENITALS
FEMALES
GONADS
HORMONES
HYDROXY COMPOUNDS
HYPOTHESIS
INJECTION
INTAKE
IONIZING RADIATIONS
KETONES
MAMMALS
MEDICINE
ORGANIC COMPOUNDS
ORGANS
OVARIES
PREGNANES
PROGESTERONE
PROSTAGLANDINS
RADIATION EFFECTS
RADIATIONS
RUMINANTS
SHEEP
STEROID HORMONES
STEROIDS
SURGERY
UTERUS
VERTEBRATES
WEIGHT
X RADIATION