Effect of hydrocortisone on cell morphology in C6 cells
Hydrocortisone has been found to induce cell spreading in rat glial C6 cells by 24 hours after its addition. This spreading phenomenon is correlated with an increase in the fraction of the peripheral cytoplasm occupied by microfilaments. Cytochalasin B causes disorganization of microfilaments in the peripheral cytoplasm of the cells. Additionally, it also prevents cell spreading in response to hormonal stimulation. High levels of calcium prevent recovery of normal microfilament organization and cell spreading following removal of cytochalasin B, but have no effect on normal microfilament organization alone. Additionally both the hydrocortisone induced spreading of C6 cells and increases in peripheral microfilaments are shown to be dependent on RNA and protein synthesis. The levels of protein co-electrophorescing with actin are not affected by hydrocortisone.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 7208242
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 94:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL CONSTITUENTS
ULTRASTRUCTURAL CHANGES
HYDROCORTISONE
BIOLOGICAL EFFECTS
ACTIN
CALCIUM
CELL CULTURES
CYTOPLASM
IN VITRO
PROTEINS
RATS
RNA
SYNTHESIS
ADRENAL HORMONES
ALKALINE EARTH METALS
ANIMALS
CORTICOSTEROIDS
ELEMENTS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
METALS
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PREGNANES
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550000* - Biomedical Sciences
Basic Studies
550800 - Morphology