Solid phase radiosyntheses for antibody labeling; Annual progress report for period July 15, 1989--July 14, 1990
The overall objective of this project is to develop a rapid, quantitative, and site specific method of radiolabeling monoclonal antibodies using a solid phase system. We describe two methods for radiolabeling the Fc portion of an antibody, each of which results in a very high specific activity radiolabeled product and, at the same time, preserves the immunologically specific Fab portion of antibody. The first method involves adding a thiophene derivative to the sugar residues of the Fc portion of antibody and linking this to Sephrose 6B resin. Iodination of the resin-linked antibody results in the release of iodinated antibody; uniodinated antibody remains bound to the resin. The second method involves adjusting the pH of an antibody solution so that only lysine residues in the Fc portion react with thiophene. The thiophene modified antibody is then coupled to a resin and iodinated with the subsequent release of only iodinated antibody. Work is being initiated on a chelating agent to allow solid phase systems to be used for the radiolabeling of the monoclonals with isotopes such as indium-111 and yttrium-90.
- Research Organization:
- Pittsburgh Univ., PA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-89ER60869
- OSTI ID:
- 720467
- Report Number(s):
- DOE/ER/60869-1; ON: TI90020747
- Resource Relation:
- Other Information: PBD: Feb 1990
- Country of Publication:
- United States
- Language:
- English
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