1. 5-Mb YAC contig in Xq28 formatted with sequence-tagged sites and including a region ustable in the clones

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1234· OSTI ID:7200304

Palmieri, G; Romano, G; Casamassimi, A; D'Urso, ^[1]; Little, R D; Abidi, F E; Schlessinger, D ^[2]; Lagerstroem, M; Malmgren, H; Steen-Bondeson, M L ^[3]

---

1. International Institute of Genetics and Biophysics, Naples (Italy)
2. Washington Univ., St. Louis, MO (United States)
3. and others

A contig of 20 yeast artificial clones (YACs) has been assembled across 1.5 Mb of Xq28 and formatted with nine previously reported probes and nine STSs developed from the sequence of probes and end fragments of YACs. YAC end fragments were obtained by subcloning, Alu-vector PCR, or primer-ligation PCR methods. Eighteen of the YACs were recovered from a library specific for Xq24-q28; two that fill a gap were obtained from a second library made from total human DNA. One region, containing probes pX78c and 2A1.1, was unstable in YACs, but it was possible to generate a self-consistent map of DNA over the entire contig. Overlaps were confirmed by Southern blot analyses of YAC DNAs, and pulsed-field gel electrophoresis confirmed the extent of the contig and identified at least four CpG islands in the region. 27 refs., 3 figs., 3 tabs.

• OSTI ID: 7200304
• Journal Information: Genomics; (United States), Vol. 16:3; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CONTIGS
CONSTRUCTION
HUMAN X CHROMOSOME
GENETIC MAPPING
CHROMOSOMES
DNA
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
X CHROMOSOME
550400* - Genetics