Evidence for lens oxoprolinase, an enzyme of the gamma-glutamyl cycle. [Rabbits]

Journal Article · Sat Mar 01 04:00:00 EDT 1975 · Invest. Ophthalmol.; (United States)

OSTI ID:7199288

Reddy, V N; Chakrapani, B; Rathbun, W B; Hough, M M

The presence of oxoprolinase, an enzyme of the ..gamma..-glutamyl cycle, not previously reported in the lens was demonstrated by organ-culture technique and from a study of the partially purified enzyme. The evidence for oxoprolinase in intact rabbit lens is based on the following: (1) (/sup 14/C)-labeled oxoproline is utilized by the lens giving rise to labeled CO/sub 2/, (2) (/sup 14/C)-oxoproline is converted to glutamic acid, which is subsequently incorporated into glutathione, (3) formation of labeled glutamic acid and CO/sub 2/ from (/sup 14/C)-oxoproline is effectively blocked by a structural analog of the compound L-2-imidazolidone-4-carboxylic acid, a known inhibitor of oxoprolinase. The enzyme was partially purified from bovine lens capsule epithelium and certain of its properties were examined. Ocular lens was also found to contain significant amounts of oxoproline, an intermediate of the ..gamma..-glutamyl cycle. (auth)

• OSTI ID: 7199288
• Journal Information: Invest. Ophthalmol.; (United States), Journal Name: Invest. Ophthalmol.; (United States) Vol. 14:3; ISSN INOPA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATTLE
CRYSTALLINE LENS
DOMESTIC ANIMALS
ENZYMES
EYES
GLUTAMIC ACID
GLUTAMINE
GLUTATHIONE
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RABBITS
RUMINANTS
SENSE ORGANS
SYNTHESIS
TISSUE CULTURES
VERTEBRATES