Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Applied and Environmental Microbiology; (United States)

OSTI ID:7199045

Raskin, L; Poulsen, L K; Noguera, D R; Stahl, D A ^[1]; Rittmann, B E ^[2]

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1. Univ. of Illinois, Urbana-Champaign, IL (United States)
2. Northwestern Univ., Evanston, IL (United States)

The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of [sup 32]P- and fluorescent-dye-labeled probes to the 16S rRNAs from samples taken from laboratory acetate-fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order methanobacterials, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively. 57 refs., 4 figs., 1 tab.

• OSTI ID: 7199045
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 60:4; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANAEROBIC CONDITIONS
BACTERIA
BIOREACTORS
DNA HYBRIDIZATION
EVALUATION
HYBRIDIZATION
METHANOGENIC BACTERIA
MICROORGANISMS