Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high-performance liquid chromatography
Journal Article
·
· Journal of Chromatography (International Journal on Chromatography, Electrophoresis and Related Methods); (Netherlands)
- Pacific Northwest Lab., Richland, WA (United States)
Previous studies have failed to provide an acceptable mass balance for 2,4,6-trinitrotoluene (TNT) in soils and plants due to deficiencies in analytical methodology. A high-performance liquid chromatographic (HPLC) method for soil analysis is reported which allowed for a mass balance in excess of 88% during a 2-month study. A method for plant analysis was developed which involved fractionation of organic extracts on Florisil adsorbent, to remove interfering pigments, followed by HPLC analysis of TNT and the primary metabolites, 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene. Chromatographic recovery of TNT from spiked tissues was 85{plus minus}6%. The methodology was utilized to investigate TNT uptake and metabolism in plants grown in TNT hydroponic solutions.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7198183
- Journal Information:
- Journal of Chromatography (International Journal on Chromatography, Electrophoresis and Related Methods); (Netherlands), Journal Name: Journal of Chromatography (International Journal on Chromatography, Electrophoresis and Related Methods); (Netherlands) Vol. 518; ISSN 0021-9673; ISSN JOCRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
54 ENVIRONMENTAL SCIENCES
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
BODY
CHEMICAL ANALYSIS
CHEMICAL EXPLOSIVES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DECOMPOSITION
ENVIRONMENTAL TRANSPORT
EXPERIMENTAL DATA
EXPLOSIVES
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MASS BALANCE
MASS TRANSFER
METABOLITES
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT TISSUES
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SOILS
TISSUES
TNT
UPTAKE
400105 -- Separation Procedures
54 ENVIRONMENTAL SCIENCES
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
BODY
CHEMICAL ANALYSIS
CHEMICAL EXPLOSIVES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DECOMPOSITION
ENVIRONMENTAL TRANSPORT
EXPERIMENTAL DATA
EXPLOSIVES
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MASS BALANCE
MASS TRANSFER
METABOLITES
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT TISSUES
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SOILS
TISSUES
TNT
UPTAKE