Genetics and complementation of Haemophilus influenzae mutants deficient in adenosine 5'-triphosphate-dependent nuclease

Journal Article · Thu Apr 01 04:00:00 EST 1976 · J. Bacteriol.; (United States)

OSTI ID:7196852

Kooistra, J; Small, G D; Setlow, J K; Shapanka, R

Eight different mutations in Haemophilus influenzae leading to deficiency in adenosine 5'-triphosphate (ATP)-dependent nuclease have been investigated in strains in which the mutations of the originally mutagenized strains have been transferred into the wild type. Sensitivity to mitomycin C and deoxycholate and complementation between extracts and deoxyribonucleic acid (DNA)-dependent ATPase activity have been measured. Genetic crosses have provided information on the relative position of the mutations on the genome. There are three complementation groups, corresponding to three genetic groups. The strains most sensitive to mitomycin and deoxycholate, derived from mutants originally selected on the basis of sensitivity to mitomycin C or methyl methanesulfonate, are in one group. Apparently all these sensitive strains lack DNA-dependent ATPase activity, as does a strain intermediate in sensitivity to deoxycholate, which is the sole representative of another group. There are four strains that are relatively resistant to deoxycholate and mitomycin C, and all of these contain the ATPase activity.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7196852
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 126:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKANES
ANTIBIOTICS
ANTIMITOTIC DRUGS
ATP
ATP-ASE
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGY
CRYOGENIC FLUIDS
DNA
DRUGS
ENZYMES
ESTERASES
ESTERS
FLUIDS
GENETICS
HAEMOPHILUS
HYDROCARBONS
HYDROLASES
METHANE
MICROORGANISMS
MITOMYCIN
MUTANTS
MUTATIONS
NUCLEASES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHATASES
PHOSPHOTRANSFERASES
SULFONIC ACID ESTERS
TRANSFERASES