Effect of disalicylidenepropanediamine on the light-dependent reduction of carbon dioxide and glycerate 3-phosphate in intact spinach chloroplasts

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.55.1.12· OSTI ID:7196849

Robinson, J M; Latzko, E; Gibbs, M

Disalicylidenepropanediamine (DSPD) at 0.1 to 1 mM levels inhibited light-dependent /sup 14/CO/sub 2/ assimilation in intact spinach chloroplasts about 50 to 80 percent, and this inhibition was accompanied by an increased ratio of /sup 14/C-glycerate 3-phosphate to /sup 14/C-glyceraldehyde 3-phosphate. Enzymatic analysis established that DSPD also inhibited the light-dependent reduction of glycerate 3-phosphate in intact spinach chloroplasts. DSPD at 0.5 mM did not inhibit ribose 5-phosphate isomerase, ribulose 5-phosphate kinase, glycerate 3-phosphate kinase, NADP/sup +/-linked glyceraldehyde 3-phosphate dehydrogenase or ribulose 1,5-diphosphate carboxylase. The inhibition of chloroplast /sup 14/CO/sub 2/ assimilation by DSPD appeared to be related to the inhibition of the photosynthetic electron transport chain. These observations are consistent with experimental results which demonstrated that DSPD inhibited directly the chloroplast lamellar membrane-mediated, light-dependent reduction of ferredoxin. (auth)

• Research Organization: Brandeis Univ., Waltham, MA
• OSTI ID: 7196849
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 55:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKANES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBON 14
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CHLOROPLASTS
EVEN-EVEN NUCLEI
FOOD
GLYCERIC ACID
HYDROCARBONS
HYDROXY ACIDS
INHIBITION
IRON COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PROPANE
RADIOISOTOPES
REACTION KINETICS
SALICYLIC ACID
SPINACH
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS
VEGETABLES
YEARS LIVING RADIOISOTOPES