Effect of disalicylidenepropanediamine on the light-dependent reduction of carbon dioxide and glycerate 3-phosphate in intact spinach chloroplasts
Disalicylidenepropanediamine (DSPD) at 0.1 to 1 mM levels inhibited light-dependent /sup 14/CO/sub 2/ assimilation in intact spinach chloroplasts about 50 to 80 percent, and this inhibition was accompanied by an increased ratio of /sup 14/C-glycerate 3-phosphate to /sup 14/C-glyceraldehyde 3-phosphate. Enzymatic analysis established that DSPD also inhibited the light-dependent reduction of glycerate 3-phosphate in intact spinach chloroplasts. DSPD at 0.5 mM did not inhibit ribose 5-phosphate isomerase, ribulose 5-phosphate kinase, glycerate 3-phosphate kinase, NADP/sup +/-linked glyceraldehyde 3-phosphate dehydrogenase or ribulose 1,5-diphosphate carboxylase. The inhibition of chloroplast /sup 14/CO/sub 2/ assimilation by DSPD appeared to be related to the inhibition of the photosynthetic electron transport chain. These observations are consistent with experimental results which demonstrated that DSPD inhibited directly the chloroplast lamellar membrane-mediated, light-dependent reduction of ferredoxin. (auth)
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 7196849
- Journal Information:
- Plant Physiol.; (United States), Vol. 55:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINES
BIOLOGICAL EFFECTS
CHLOROPLASTS
BIOCHEMICAL REACTION KINETICS
SPINACH
PHOTOSYNTHESIS
CARBON 14
CARBON DIOXIDE
GLYCERIC ACID
INHIBITION
IRON COMPOUNDS
ORGANIC COMPOUNDS
PHOSPHATES
PROPANE
SALICYLIC ACID
ALKANES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON COMPOUNDS
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
EVEN-EVEN NUCLEI
FOOD
HYDROCARBONS
HYDROXY ACIDS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
OXIDES
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS
VEGETABLES
YEARS LIVING RADIOISOTOPES
550500* - Metabolism
550200 - Biochemistry