Fluorescence-detected magnetic circular dichroism of fluorescent and nonfluorescent molecules

Journal Article · Sat Jan 31 23:00:00 EST 1976 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.73.2.276· OSTI ID:7196680

Sutherland, J C; Low, H

A spectrometer was modified for the measurement of fluorescence-detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in which the average and polarization-induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe(III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission-detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent and nonfluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter-or intramolecular energy transfer.

• Research Organization: Univ. of California, Irvine
• OSTI ID: 7196680
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 73:2; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

640302* -- Atomic
Molecular & Chemical Physics-- Atomic & Molecular Properties & Theory
74 ATOMIC AND MOLECULAR PHYSICS
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
CYTOCHROMES
DICHROISM
ELEMENTS
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
IRON
MAGNETIC PROPERTIES
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYSICAL PROPERTIES
PIGMENTS
PYRROLES
SPECTROSCOPY
TRANSITION ELEMENTS
TRYPTOPHAN